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KMID : 0361020010440040388
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Korean Journal of Otolaryngology - Head and Neck Surgery
2001 Volume.44 No. 4 p.388 ~ p.391
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Expression of MUC5AC mRNA in the Goblet Cells of Human Nasal Mucosa
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강성석/Seong Seok Kang
김창훈/김현웅/이정권/윤주헌/Chang Hoon Kim/Hyun Ung Kim/Jeung Gweon Lee/Joo Heon Yoon
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Abstract
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Backgrounds and Objectives : Mucus hypersecretion is a common feature in chronic sinusitis with polyps. Since mucus hypersecretion is commonly accompanied by goblet cell hyperplasia, it is important to identify which mucin gene mRNAs are
expressed
in
the goblet cells of the surface epithelium in the human airway. This study aims to investigate the pattern of expression of MUC5AC mRNA in the goblet cells of human nasal mucosa. Materials and methods : Six nasal polyps, five inferior turbinate
mucosa
specimens, and three normal-appearing mucosa specimens of the posterior ethmoid sinus were obtained. Each of the specimens were cut into 10 ㎛-thick serial frozen sections and in situ hybridization of MUC5AC mRNA was performed with an
oligonucleotide
probe. Alcian blue(pH 2.5)-periodic acid-Schiff staining was performed on the serial sections. Results : In human nasal polyps, MUC5AC mRNA was expressed in the cytoplasm of most of the goblet cells. However, in the inferior turbinate, MUC5AC
mRNA
was
expressed in only some of the goblet cells. On the contrary, in the normal appearing mucosa of the posterior ethmoid sinus, MUC5AC mRNA was barely expressed in the goblet cells. Furthermore, MUC5AC mRNA was mainly expressed in some of the
PAS-positive
goblet cells. Conclusion : Only a portion of the goblet cells in the human nasal mucosa expressed MUC5AC mRNA. This result suggests that surface goblet cells might have other mucin genes in addition to MUC2 and MUC5AC.
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KEYWORD
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